Program

Biomacromolecular complexes and assemblies (protein-RNA/DNA complexes, e.g., viruses, ribosomes; quaternary protein structures; DNA, proteins, polyelectrolytes). The lecturers will describe related problems and discuss approaches to solutions, e.g.:

• spectroscopies (NMR, EPR, FTIR, Raman, MS…)
• microscopies (AFM, fluorescence techniques, tweezers…)
• diffraction methods
• computational solutions, modeling and simulations

PROGRAM >>>

Lecturers

• Nenad Ban, ETH, Zürich, Switzerland: Protein structure& function; X-ray diffraction
  • Ribosomes and their functional complexes
  • Beyond the prokaryotic ribosome
• Jasna Brujic, NYU, New York, USA: Soft matter, statistical physics; single molecule experiments-AFM
  • Emulsions and packing
  • Single-molecule AFM studies
• Mario Cindrić, Ruđer Bošković Institute
  • Mass Spectrometry for protein sequencing
• Yves Engelborghs, KU Leuven, Belgium: Fluorescence microscopy and spectroscopy
  • Time resolved fluorescence spectroscopy of proteins
  • Fluorescence Correlation Spectroscopy : an elegant way to study molecular interactions.
• Helmut Grubmueller (COST lecturer), MPI-BPC, Göttingen, Germany: Theoretical and Computational Biophysics
  • Forces and Conformational Dynamics in Biomolecular Nanomachines 1,2
• Alexei Kedrov,  Gene Center LMU
  • Biophysical analysis on membrane protein targeting and assembly
• Jané Kondev, Brandeis University, USA
  • Chromosome Folding in Cells
  • Transcriptional Regulation
• Pierre-Emmanuel Milhiet, Centre de Biochimie Structurale, Montpellier, France: High-performance AFM, membrane proteins imaging
  • Near field microscopies in structural biology
    
  • AFM imaging in the field of biological membranes
  • New developments in Bio-AFM imaging
• Lennart Nilsson, Karolinska Institutet, Sweden: Molecular modeling, nucleic acids, peptides and their interactions
• Chris Oostenbrink, UNRLF, Vienna, Austria: Structure and dynamics of biomolecules; modeling
  • Ensembles and sampling, leading to molecular dynamics simulations
  • Structure refinement using molecular dynamics simulations (NMR observables)
  • Calculation of free energies from molecular simulation
• Frances Separovic, University of Melbourne, Australia: NMR and Structural Studies of Membrane-Active Peptides
  • Solid-state NMR of membrane-active peptides
  • Membrane interactions of antimicrobial and amyloid peptides
• Ana-Sunčana Smith, Ruđer Bošković Institute & Uni Erlangen-Nürnberg Germany: Membranes and vesicles, cell adhesion
  • Membranes, from self assembly to rafts 1,2,3
• Holger Stark, MPI-BPC, Göttingen, Germany: Protein structure& function; cryoEM
  • Introduction into single particle cryo-EM
  • High-resolution structure determination of macromolecular complexes by cryo-EM
• Heinz-Jürgen Steinhoff (COST lecturer), University of Osnabrück, Germany: Macromolecular Structure, EPR
  • Principles of site-directed spin labeling EPR
  • Site-directed spin labeling EPR of biomacromolecules,  complexes and assemblies
• Antonio Šiber, Institute of physics, Zagreb, Croatia: Physics of viruses
  • Physics of viruses: electrostatics, elasticity and DNA condensation in viruses 1,2,3
• Iva Tolić, Ruđer Bošković Institute & MPI-CBG, Dresden, Germany & RBI, Zagreb: Physics of cells, fluorescence-microscopy
  • Microtubules and motor proteins 1, 2
• Piotr Wardega, Nanotemper GmbH
  • Quantitative analysis of biomolecular interactions with Microscale Thermophoresis (MST)
• Anthony Watts, Department of Biochemistry, Oxford, UK: Protein structure& function; solid state NMR
  • Principles of solid state NMR for the study of biomolecules
  • Solid state NMR for structural studies of large integral membrane proteins
  • Receptor dynamics and structure in membranes resolved using solid state NMR
• Bojan Žagrović, MFPL, Vienna, Austria: Computational Biophysics of Macromolecules
  • More dynamic than we think?  On conformational averaging in structural biology 1, 2
  • Protein-RNA interactions and the origin of the genetic code